Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.

ABSTRACT: We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7053107 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature