Integrative proteomic and glycoproteomic profiling of Mycobacterium tuberculosis culture filtrate.

ABSTRACT: Despite being the subject of intensive research, tuberculosis, caused by Mycobacterium tuberculosis, remains at present the leading cause of death from an infectious agent. Secreted and cell wall proteins interact with the host and play important roles in pathogenicity. These proteins are explored as candidate diagnostic markers, potential drug targets or vaccine antigens, and more recently special attention is being given to the role of their post-translational modifications. With the purpose of contributing to the proteomic and glycoproteomic characterization of this important pathogen, we performed a shotgun analysis of culture filtrate proteins of M. tuberculosis based on a liquid nano-HPLC tandem mass spectrometry and a label-free spectral counting normalization approach for protein q

SUBMITTER: Tucci P 

PROVIDER: S-EPMC7053730 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature