A space-saving visual screening method, Glycine max FAST, for generating transgenic soybean.

ABSTRACT: Establishing homozygous transgenic lines of Glycine max is time-consuming and laborious. To overcome the difficulties, we developed a powerful method for selecting transgenic soybean plants, Fluorescence-Accumulating Seed Technology (GmFAST). GmFAST uses a marker composed of a soybean seed-specific promoter coupled to the OLE1-GFP gene, which encodes a GFP fusion of the oil-body membrane protein OLEOSIN1 of Arabidopsis thaliana. We introduced the marker gene into cotyledonary nodes of G. max Kariyutaka via Agrobacterium-mediated transformation and regenerated heterozygous transgenic plants. OLE1-GFP-expressing soybean seeds can be selected nondestructively with a fluorescence stereomicroscope. Among T₂ seeds, the most strongly fluorescent seeds were h

SUBMITTER: Iwabuchi K 

PROVIDER: S-EPMC7053950 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature