Promotion of Hyperthermic-Induced rDNA Hypercondensation in Saccharomyces cerevisiae.

ABSTRACT: Ribosome biogenesis is tightly regulated through stress-sensing pathways that impact genome stability, aging and senescence. In Saccharomyces cerevisiae, ribosomal RNAs are transcribed from rDNA located on the right arm of chromosome XII. Numerous studies reveal that rDNA decondenses into a puff-like structure during interphase, and condenses into a tight loop-like structure during mitosis. Intriguingly, a novel and additional mechanism of increased mitotic rDNA compaction (termed hypercondensation) was recently discovered that occurs in response to temperature stress (hyperthermic-induced) and is rapidly reversible. Here, we report that neither changes in condensin binding or release of DNA during mitosis, nor mutation of factors that regulate cohesin binding and release, appear to

SUBMITTER: Shen D 

PROVIDER: S-EPMC7054013 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature