Impact of Chromosome Fusions on 3D Genome Organization and Gene Expression in Budding Yeast.

ABSTRACT: The three-dimensional (3D) organization of chromosomes can influence transcription. However, the frequency and magnitude of these effects remain debated. To determine how changes in chromosome positioning affect transcription across thousands of genes with minimal perturbation, we characterized nuclear organization and global gene expression in budding yeast containing chromosome fusions. We used computational modeling and single-cell imaging to determine chromosome positions, and integrated these data with genome-wide transcriptional profiles from RNA sequencing. We find that chromosome fusions dramatically alter 3D nuclear organization without leading to strong genome-wide changes in transcription. However, we observe a mild but significant and reproducible increase in the expression of

SUBMITTER: Di Stefano M 

PROVIDER: S-EPMC7054015 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature