A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer.

ABSTRACT: Light-sensitive proteins can be used to perturb signaling networks in living cells and animals with high spatiotemporal resolution. We recently engineered a protein heterodimer that dissociates when irradiated with blue light and demonstrated that by fusing each half of the dimer to termini of a protein that it is possible to selectively block binding surfaces on the protein when in the dark. On activation with light, the dimer dissociates and exposes the binding surface, allowing the protein to bind its partner. Critical to the success of this system, called Z-lock, is that the linkers connecting the dimer components to the termini are engineered so that the dimer forms over the appropriate binding surface. Here, we develop and test a protocol in the Rosetta molecular modeling program for

SUBMITTER: Teets FD 

PROVIDER: S-EPMC7054171 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature