Generation of a conditional Flpo/FRT mouse model expressing constitutively active TGFβ in fibroblasts.

ABSTRACT: Transforming growth factor (TGFβ) is a secreted factor, which accumulates in tissues during many physio- and pathological processes such as embryonic development, wound healing, fibrosis and cancer. In order to analyze the effects of increased microenvironmental TGFβ concentration in vivo, we developed a conditional transgenic mouse model (Flpo/Frt system) expressing bioactive TGFβ in fibroblasts, a cell population present in the microenvironment of almost all tissues. To achieve this, we created the genetically-engineered [Fsp1-Flpo; ^FSFTGFβ^CA] mouse model. The Fsp1-Flpo allele consists in the Flpo recombinase under the control of the Fsp1 (fibroblast-specific promoter 1) promoter. The ^FSFTGFβ^CA allele consists in a transgene encoding a constitu

SUBMITTER: Cardot-Ruffino V 

PROVIDER: S-EPMC7054254 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature