Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives.

ABSTRACT: Although human induced pluripotent stem cell (hiPSC) lines are karyotypically normal, they retain the potential for mutation in the genome. Accordingly, intensive and relevant quality controls for clinical-grade hiPSCs remain imperative. As a conceptual approach, we performed RNA-seq-based broad-range genetic quality tests on GMP-compliant human leucocyte antigen (HLA)-homozygous hiPSCs and their derivatives under postdistribution conditions to investigate whether sequencing data could provide a basis for future quality control. We found differences in the degree of single-nucleotide polymorphism (SNP) occurring in cells cultured at three collaborating institutes. However, the cells cultured at each centre showed similar trends, in which more SNPs occurred in late-passage hiPSCs than in ea

SUBMITTER: Jo HY 

PROVIDER: S-EPMC7054319 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature