In vitro Chicken Bone Marrow-Derived Dendritic Cells Comprise Subsets at Different States of Maturation.

ABSTRACT: Research in chickens has been fundamental for the discovery of basic aspects of the immune system and has led to an interest in the in-depth characterization of avian immune cell types including dendritic cells (DCs). The in vitro generation and expansion of chicken bone marrow-derived DCs (chBMDCs) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) has provided a way to study chicken DCs, which are only present at limited cell numbers in vivo. This method has been employed to study the interactions between chicken DCs and pathogens or vaccines. However, a detailed characterization of the chBMDC culture is still lacking. In the present study, we performed an elaborate phenotypical and functional analysis of the chBMDC culture and addressed its hetero

SUBMITTER: van den Biggelaar RHGA 

PROVIDER: S-EPMC7054383 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature