Transcriptional signature of prion-induced neurotoxicity in a Drosophila model of transmissible mammalian prion disease.

ABSTRACT: Prion diseases are fatal transmissible neurodegenerative conditions of humans and animals that arise through neurotoxicity induced by PrP misfolding. The cellular and molecular mechanisms of prion-induced neurotoxicity remain undefined. Understanding these processes will underpin therapeutic and control strategies for human and animal prion diseases, respectively. Prion diseases are difficult to study in their natural hosts and require the use of tractable animal models. Here we used RNA-Seq-based transcriptome analysis of prion-exposed Drosophila to probe the mechanism of prion-induced neurotoxicity. Adult Drosophila transgenic for pan neuronal expression of ovine PrP targeted to the plasma membrane exhibit a neurotoxic phenotype evidenced by decreased locomotor activity after exposure to

SUBMITTER: Thackray AM 

PROVIDER: S-EPMC7054746 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature