Chitinase 3 like 1 suppresses the stability and activity of p53 to promote lung tumorigenesis.

ABSTRACT:

Background

Chitinase 3 like 1 protein (Chi3L1) is expressed in several cancers, and a few evidences suggest that the secreted Chi3L1 contributes to tumor development. However, the molecular mechanisms of intracellular Chi3L1 are unknown in the lung tumor development.

Methods

In the present study, we generated Chi3L1 knockout mice (Chi3L1^KO(-/-)) using CRISPR/Cas9 system to investigate the role of Chi3L1 on lung tumorigenesis.

Results

We established lung metastasis induced by i.v. injections of B16F10 in Chi3L1^KO(-/-). The lung tumor nodules were significantly reduced in Chi3L1^KO(-/-) and protein levels of p53, p21, BAX, and cleaved-caspase 3 were significantly increased in Chi3L1^KO(-/-), while protein levels of cyclin E1, CD

SUBMITTER: Park KR 

PROVIDER: S-EPMC7055043 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature