Standardized GMP-compliant scalable production of human pancreas organoids.

ABSTRACT:

Background

Organoids are three-dimensional in vitro-grown cell clusters that recapitulate key features of native organs. In regenerative medicine, organoid technology represents a promising approach for the replacement of severely damaged organs, such as the pancreas in patients with type 1 diabetes. Isolation human pancreas organoids (hPOs) in chemically defined serum-free culture media would be a major milestone for this approach.

Methods

Starting from discarded pancreatic tissues, we developed a large-scale process for obtaining clinically relevant quantities of undifferentiated organoids, obviating enzymatic digestion and operator-dependent pancreatic ducts picking steps. hPO identity was characterized by molecular and flow cytometry analysis.

Results

This work de

SUBMITTER: Dossena M 

PROVIDER: S-EPMC7055108 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature