Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 Interaction.

ABSTRACT: The activation of neuronal plasma membrane Ca²⁺ channels stimulates many intracellular responses. Scaffolding proteins can preferentially couple specific Ca²⁺ channels to distinct downstream outputs, such as increased gene expression, but the molecular mechanisms that underlie the exquisite specificity of these signaling pathways are incompletely understood. Here, we show that complexes containing CaMKII and Shank3, a postsynaptic scaffolding protein known to interact with L-type calcium channels (LTCCs), can be specifically coimmunoprecipitated from mouse forebrain extracts. Activated purified CaMKIIα also directly binds Shank3 between residues 829 and 1130. Mutation of Shank3 residues ⁹⁴⁹Arg-Arg-Lys⁹⁵¹ to three alanines disrupts CaMKII binding

SUBMITTER: Perfitt TL 

PROVIDER: S-EPMC7055140 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature