High-Throughput Screening to Predict Chemical-Assay Interference.

ABSTRACT: The U.S. federal consortium on toxicology in the 21^st century (Tox21) produces quantitative, high-throughput screening (HTS) data on thousands of chemicals across a wide range of assays covering critical biological targets and cellular pathways. Many of these assays, and those used in other in vitro screening programs, rely on luciferase and fluorescence-based readouts that can be susceptible to signal interference by certain chemical structures resulting in false positive outcomes. Included in the Tox21 portfolio are assays specifically designed to measure interference in the form of luciferase inhibition and autofluorescence via multiple wavelengths (red, blue, and green) and under various conditions (cell-free and cell-based, two cell types). Out of 8,305 chemicals tested in

SUBMITTER: Borrel A 

PROVIDER: S-EPMC7055224 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature