Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization.

ABSTRACT: Small-angle neutron scattering (SANS) is one of the most widely used neutron-based approaches to study the solution structure of biological macromolecular systems. The selective deuterium labelling of different protein components of a complex provides a means to probe conformational changes in multiprotein complexes. The Lysinibacillus sphaericus mosquito-larvicidal BinAB proteins exert toxicity through interaction with the receptor Cqm1 protein; however, the nature of the complex is not known. Rationally engineered deuterated BinB (dBinB) protein from the L. sphaericus ISPC-8 species was synthesized using an Escherichia coli-based protein-expression system in M9 medium in D₂O for 'contrast-matched' SANS experiments. SANS data were independently analysed by

SUBMITTER: Sharma M 

PROVIDER: S-EPMC7055391 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature