Fiber orientation in a whole mouse heart reconstructed by laboratory phase-contrast micro-CT.

ABSTRACT: Purpose: We present a phase-contrast x-ray tomography study of wild type C57BL/6 mouse hearts as a nondestructive approach to the microanatomy on the scale of the entire excised organ. Based on the partial coherence at a home-built phase-contrast μ - CT setup installed at a liquid metal jet source, we exploit phase retrieval and hence achieve superior image quality for heart tissue, almost comparable to previous synchrotron data on the whole organ scale. Approach: In our work, different embedding methods and heavy metal-based stains have been explored. From the tomographic reconstructions, quantitative structural parameters describing the three-dimensional (3-D) architecture have been derived by two different fiber tracking algorithms. The first algorithm is based on the loc

SUBMITTER: Reichardt M 

PROVIDER: S-EPMC7055497 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature