Bone Marrow-Derived Cells Restore Functional Integrity of the Gut Epithelial and Vascular Barriers in a Model of Diabetes and ACE2 Deficiency.

ABSTRACT:

Rationale

There is incomplete knowledge of the impact of bone marrow cells on the gut microbiome and gut barrier function.

Objective

We postulated that diabetes mellitus and systemic ACE2 (angiotensin-converting enzyme 2) deficiency would synergize to adversely impact both the microbiome and gut barrier function.

Methods and results

Bacterial 16S rRNA sequencing and metatranscriptomic analysis were performed on fecal samples from wild-type, ACE2^-/y, Akita (type 1 diabetes mellitus), and ACE2^-/y-Akita mice. Gut barrier integrity was assessed by immunofluorescence, and bone marrow cell extravasation into the small intestine was evaluated by flow cytometry. In the ACE2^-/y-Akita or Akita mice, the disrupted barrier was associated with redu

SUBMITTER: Duan Y 

PROVIDER: S-EPMC7056264 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature