The virtually mature B-type natriuretic peptide (BNP1-32) is a precursor for the more effective BNP1-30.

ABSTRACT:

Background and purpose

The B-type natriuretic peptide (BNP1-32) exerts vasorelaxing and cardioprotective activity. BNP is used as a biomarker for the diagnosis of cardiopathological conditions and recombinant BNP1-32 as a drug for the treatment of such. BNP1-32 has a short half-life and thus, similar to other vasoactive peptides like angiotensin II and bradykinin, can be enzymatically truncated forming bioactive metabolites. We aimed to investigate the metabolism of BNP1-32 in the mouse lung, to identify potential new BNP metabolites and to disclose their biological activity compared to the BNP1-32, in vitro and in vivo.

Experimental approach

Using HPLC and MS, we identified a new BNP metabolite, BNP1-30, in the lung being generated by endothelin-converting enzyme-1.

Key

SUBMITTER: Schwiebs A 

PROVIDER: S-EPMC7056463 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature