Characterization of exosomes in peritoneal fluid of endometriosis patients.

ABSTRACT:

Objective

To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and characterized their cargo.

Design

Case-control experimental study.

Setting

Academic clinical center.

Patient (s)

Women with and without endometriosis who underwent laparoscopic surgery (n = 28 in total).

Intervention (s)

None.

Main outcome measure (s)

Concentration of exosomes within peritoneal fluid and protein content of the isolated exosomes.

Result (s)

Peritoneal fluid samples were pooled according to the cycle phase and disease stage to form six experimental groups, from which the exosomes were isolated. Exosomes were successfully isolated from peritoneal fluid in a

SUBMITTER: Nazri HM 

PROVIDER: S-EPMC7057257 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature