Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes.

ABSTRACT:

Background

H1t is the major linker histone variant in pachytene spermatocytes, where it constitutes 50-60% of total H1. This linker histone variant was previously reported to localize in the nucleolar rDNA element in mouse spermatocytes. Our main aim was to determine the extra-nucleolar localization of this linker histone variant in pachytene spermatocytes.

Results

We generated H1t-specific antibodies in rabbits and validated its specificity by multiple assays like ELISA, western blot, etc. Genome-wide occupancy studies, as determined by ChIP-sequencing in P20 mouse testicular cells revealed that H1t did not closely associate with active gene promoters and open chromatin regions. Annotation of H1t-bound genomic regions revealed that H1t is depleted from DSB hotspots and TSS,

SUBMITTER: Mahadevan IA 

PROVIDER: S-EPMC7057672 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature