Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation.

ABSTRACT: Flavin mononucleotide (FMN) belongs to the group of very efficient endogenous photosensitizers producing singlet oxygen, ¹O₂, but with limited ability to be targeted. On the other hand, in genetically-encoded photosensitizers, which can be targeted by means of various tags, the efficiency of FMN to produce ¹O₂ is significantly diminished due to its interactions with surrounding amino acid residues. Recently, an increase of ¹O₂ production yield by FMN buried in a protein matrix was achieved by a decrease of quenching of the cofactor excited states by weakening of the protein-FMN interactions while still forming a complex. Here, we suggest an alternative approach which relies on the blue light irradiation-induced dissociation

SUBMITTER: Petrencakova M 

PROVIDER: S-EPMC7058016 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature