The vascular glycocalyx is not a mechanosensor in conduit arteries in the anesthetized pig.

ABSTRACT:

Background

The role of the glycocalyx as the endothelial sensor of an increase in blood flow was assessed in the iliac artery in vivo.

Methods

Acetylcholine-induced flow mediated dilation was evaluated before and after vascular glycocalyx disruption. This was accomplished by exposing the iliac lumen to the chemotactic agent fMLP (1 μM; n = 6 pigs), concomitant heparinase III (100 mU ml^-1) and hyaluronidase (14 mg ml^-1) (n = 4), and neuraminidase (140 mU ml^-1; n = 5), for 20 min in separate iliac artery preparations. Only one lumen intervention per iliac was conducted.

Results

For the heparinase III + hyaluronidase experiment, the iliac diameter increased by an average of 0.54 ± 0.11 mm before and 0.45 ± 0.03 mm aft

SUBMITTER: Ruane-O'Hora T 

PROVIDER: S-EPMC7058102 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature