Recurring genomic structural variation leads to clonal instability and loss of productivity.

ABSTRACT: Chinese hamster ovary cells, commonly used in the production of therapeutic proteins, are aneuploid. Their chromosomes bear structural abnormality and undergo changes in structure and number during cell proliferation. Some production cell lines are unstable and lose their productivity over time in the manufacturing process and during the product's life cycle. To better understand the link between genomic structural changes and productivity stability, an immunoglobulin G producing cell line was successively single-cell cloned to obtain subclones that retained or lost productivity, and their genomic features were compared. Although each subclone started with a single karyotype, the progeny quickly diversified to a population with a distribution of chromosome numbers that is not distinctive f

SUBMITTER: Bandyopadhyay AA 

PROVIDER: S-EPMC7058117 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature