Single Copy Transgene Integration in a Transcriptionally Active Site for Recombinant Protein Synthesis.

ABSTRACT: For the biomanufacturing of protein biologics, establishing stable cell lines with high transgene transcription is critical for high productivity. Modern genome engineering tools can direct transgene insertion to a specified genomic locus and can potentially become a valuable tool for cell line generation. In this study, the authors survey transgene integration sites and their transcriptional activity to identify characteristics of desirable regions. A lentivirus containing destabilized Green Fluorescent Protein (dGFP) is used to infect Chinese hamster ovary cells at a low multiplicity of infection, and cells with high or low GFP fluorescence are isolated. RNA sequencing and Assay for Transposase Accessible Chromatin using sequencing data shows integration sites with high GFP expression ar

SUBMITTER: O'Brien SA 

PROVIDER: S-EPMC7058118 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature