A Pipeline for Reconstructing Somatic Copy Number Alternation's Subclonal Population-Based Next-Generation Sequencing Data.

ABSTRACT: State-of-the-art next-generation sequencing (NGS)-based subclonal reconstruction methods perform poorly on somatic copy number alternations (SCNAs), due to not only it needs to simultaneously estimate the subclonal population frequency and the absolute copy number for each SCNA, but also there exist complex bias and noise in the tumor and its paired normal sequencing data. Both existing NGS-based SCNA detection methods and SCNA's subclonal population frequency inferring tools use the read count on radio (RCR) of tumor to its paired normal as the key feature of tumor sequencing data; however, the sequencing error and bias have great impact on RCR, which leads to a large number of redundant SCNA segments that make the subsequent process of SCNA's subclonal population frequency inferring and

SUBMITTER: Chu Y 

PROVIDER: S-EPMC7058119 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature