The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to-zygote transition.

ABSTRACT: Following fertilization of a mature oocyte, the formation of a diploid zygote involves a series of coordinated cellular events that ends with the first embryonic mitosis. In animals, this complex developmental transition is almost entirely controlled by maternal gene products. How such a crucial transcriptional program is established during oogenesis remains poorly understood. Here, we have performed an shRNA-based genetic screen in Drosophila to identify genes required to form a diploid zygote. We found that the Lid/KDM5 histone demethylase and its partner, the Sin3A-HDAC1 deacetylase complex, are necessary for sperm nuclear decompaction and karyogamy. Surprisingly, transcriptomic analyses revealed that these histone modifiers are required for the massive transcriptional activation of dea

SUBMITTER: Torres-Campana D 

PROVIDER: S-EPMC7058283 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature