Differentiation of human colon tissue in culture: Effects of calcium on trans-epithelial electrical resistance and tissue cohesive properties.

ABSTRACT:

Background and aims

Human colonoid cultures maintained under low-calcium (0.25 mM) conditions undergo differentiation spontaneously and, concomitantly, express a high level of tight junction proteins, but not desmosomal proteins. When calcium is included to a final concentration of 1.5-3.0 mM (provided either as a single agent or as a combination of calcium and additional minerals), there is little change in tight junction protein expression but a strong up-regulation of desmosomal proteins and an increase in desmosome formation. The aim of this study was to assess the functional consequences of calcium-mediated differences in barrier protein expression.

Methods

Human colonoid-derived epithelial cells were interrogated in transwell culture under low- or high-calcium conditio

SUBMITTER: McClintock SD 

PROVIDER: S-EPMC7058309 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature