Hinge length contributes to the phagocytic activity of HIV-specific IgG1 and IgG3 antibodies.

ABSTRACT: Antibody functions such as neutralization require recognition of antigen by the Fab region, while effector functions are additionally mediated by interactions of the Fc region with soluble factors and cellular receptors. The efficacy of individual antibodies varies based on Fab domain characteristics, such as affinity for antigen and epitope-specificity, and on Fc domain characteristics that include isotype, subclass, and glycosylation profile. Here, a series of HIV-specific antibody subclass and hinge variants were constructed and tested to define those properties associated with differential effector function. In the context of the broadly neutralizing CD4 binding site-specific antibody VRC01 and the variable loop (V3) binding antibody 447-52D, hinge truncation and extension had a consid

SUBMITTER: Chu TH 

PROVIDER: S-EPMC7058349 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature