Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

ABSTRACT: Succinylation is a post-translational modification of protein lysine residues with succinyl groups derived from succinyl CoA. Succinylation is considered a significant post-translational modification with the potential to impact protein function which is highly conserved across numerous species. The role of succinylation in the heart, especially in heart failure and myofibril mechanics, remains largely unexplored. Mechanical parameters were measured in myofibrils isolated from failing hearts of ischemic cardiomyopathy patients and non-failing donor controls. We employed mass spectrometry to quantify differential protein expression in myofibrils from failing ischemic cardiomyopathy hearts compared to non-failing hearts. In addition, we combined peptide enrichment by immunoprecipitation with

SUBMITTER: Ali HR 

PROVIDER: S-EPMC7058372 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature