Age-dependent expression profiles of two adaptogenic systems and thermotolerance in Drosophila melanogaster.

ABSTRACT: Here, we monitored the expression of three genes (hsp70, hsp22, and hsf1) involved in heat shock response in Drosophila melanogaster in males and females of different age. Also, we investigated age- and sex-dependent expression of three major genes participating in the production of hydrogen sulfide (H₂S) (cse, cbs, and mst), implicated in stress resistance and aging. In addition to the control strain, we monitored the expression of all of these genes in a cbs knockout strain (cbs-/-) generated using the CRISPR technique. The tested strains differ in the induction capacities of the studied genes. Relative to the control strain, under normal conditions, the cbs-/- strain expresses all of the studied genes more abundantly, especially hsp22. In the control strain, aging leads to a

SUBMITTER: Shilova V 

PROVIDER: S-EPMC7058767 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature