Inhibition of miR-153 ameliorates ischemia/reperfusion-induced cardiomyocytes apoptosis by regulating Nrf2/HO-1 signaling in rats.

ABSTRACT:

Background

Previous in vitro studies demonstrated that suppression of microRNAs might protect cardiomyocytes and neurons against oxygen-glucose deprivation and reoxygenation (OGD/R)-induced cell apoptosis. However, whether the protective effect of miR-153-inhibition on cardiomyocytes can be observed in the animal model is unknown. We aimed to address this question using a rat model of ischemia-reperfusion (I/R).

Methods

Rats were received the intramyocardial injection of saline or adenovirus-carrying target or control gene, and the rats were subjected to ischemia/reperfusion (I/R) treatment. The effects of miR-153 on I/R-induced inflammatory response and oxidative stress in the rat model were assessed using various assays.

Results

We found that suppression of miR-153

SUBMITTER: Hou W 

PROVIDER: S-EPMC7059292 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature