Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems.

ABSTRACT: BACKGROUND:The genome-integrated T7 expression system offers significant advantages, in terms of productivity and product quality, even when expressing the gene of interest (GOI) from a single copy. Compared to plasmid-based expression systems, this system does not incur a plasmid-mediated metabolic load, and it does not vary the dosage of the GOI during the production process. However, long-term production with T7 expression system leads to a rapidly growing non-producing population, because the T7 RNA polymerase (RNAP) is prone to mutations. The present study aimed to investigate whether two σ70 promoters, which were recognized by the Escherichia coli host RNAP, might be suitable in genome-integrated expression systems. We applied a promoter engineering strategy that allowed control of e

SUBMITTER: Schuller A 

PROVIDER: S-EPMC7059391 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature