Genetic barcoding reveals clonal dominance in iPSC-derived mesenchymal stromal cells.

ABSTRACT:

Background

The use of mesenchymal stromal cells (MSCs) for research and clinical application is hampered by cellular heterogeneity and replicative senescence. Generation of MSC-like cells from induced pluripotent stem cells (iPSCs) may circumvent these limitations, and such iPSC-derived MSCs (iMSCs) are already tested in clinical trials. So far, a comparison of MSCs and iMSCs was particularly addressed in bulk culture. Despite the high hopes in cellular therapy, only little is known how the composition of different subclones changes in these cell preparations during culture expansion.

Methods

In this study, we used multicolor lentiviral genetic barcoding for the marking of individual cells within cell preparations. Based on this, we could track the clonal composition of syng

SUBMITTER: Hollmann J 

PROVIDER: S-EPMC7059393 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature