The pivotal role of micro-environmental cells in a human blood-brain barrier in vitro model of cerebral ischemia: functional and transcriptomic analysis.

ABSTRACT: BACKGROUND:The blood-brain barrier (BBB) is altered in several diseases of the central nervous system. For example, the breakdown of the BBB during cerebral ischemia in stroke or traumatic brain injury is a hallmark of the diseases' progression. This functional damage is one key event which is attempted to be mimicked in in vitro models. Recent studies showed the pivotal role of micro-environmental cells such as astrocytes for this barrier damage in mouse stroke in vitro models. The aim of this study was to evaluate the role of micro-environmental cells for the functional, paracellular breakdown in a human BBB cerebral ischemia in vitro model accompanied by a transcriptional analysis. METHODS:Transwell models with human brain endothelial cell line hCMEC/D3 in mono-culture or co-culture wit

SUBMITTER: Gerhartl A 

PROVIDER: S-EPMC7059670 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature