Towards single-cell ionomics: a novel micro-scaled method for multi-element analysis of nanogram-sized biological samples.

ABSTRACT:

Background

To understand processes regulating nutrient homeostasis at the single-cell level there is a need for new methods that allow multi-element profiling of biological samples ultimately only available as isolated tissues or cells, typically in nanogram-sized samples. Apart from tissue isolation, the main challenges for such analyses are to obtain a complete and homogeneous digestion of each sample, to keep sample dilution at a minimum and to produce accurate and reproducible results. In particular, determining the weight of small samples becomes increasingly challenging when the sample amount decreases.

Results

We developed a novel method for sampling, digestion and multi-element analysis of nanogram-sized plant tissue, along with strategies to quantify element concent

SUBMITTER: Chen A 

PROVIDER: S-EPMC7059671 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature