LncRNA MALAT1 silencing protects against cerebral ischemia-reperfusion injury through miR-145 to regulate AQP4.

ABSTRACT: BACKGROUND:The present study aimed to verify whether long noncoding RNA (lncRNA) MALAT1 is involved in brain tissue damage induced by ischemia-reperfusion injury, and to explore the mechanism by which MALAT1 regulates aquaporin 4 (AQP4). METHODS:In this study, we established glucose deprivation (OGD)/reoxygenation (RX) astrocyte cell model and middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vitro and in vivo. Then cell counting kit-8 assay, flow cytometry analysis, Triphenyltetrazolium chloride (TTC) staining, and western blotting were used to determine cell viability, cell apoptosis, cerebral infarction volume, and the abundance of AQP4, respectively. RESULTS:We found that the level of MALAT1 was significantly upregulated in both the MCAO/reperfusion model and OGD/RX mo

SUBMITTER: Wang H 

PROVIDER: S-EPMC7059719 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature