Phosphatidylinositol 3-kinase-δ controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice.

ABSTRACT:

Background and purpose

Phosphatidylinositol 3-kinase (PI3K), especially PI3K-δ, and endoplasmic reticulum (ER) stress play important roles in refractory asthma induced by the fungus Aspergillus fumigatus through mechanisms that are not well understood. Here we have investigated these mechanisms, using BEAS-2B human bronchial epithelial cells and a mouse model of A. fumigatus-induced allergic lung inflammation.

Experimental approach

A selective PI3K-δ inhibitor, IC87114, and an ER folding chaperone, 4-phenylbutyric acid (4-PBA), were applied to a model of A. fumigatus-induced asthma in female C57BL/6 mice. The therapeutic potential of IC87114 and 4-PBA was assessed in relevant primary cell, tissue, and disease models, using immunohistochemistry, western blotting and assessmen

SUBMITTER: Lee HY 

PROVIDER: S-EPMC7060358 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature