Morphine-induced RACK1-dependent autophagy in immortalized neuronal cell lines.

ABSTRACT:

Background and purpose

Autophagy is a critical cellular catabolic process in cell homoeostasis and brain function. Recent studies indicate that receptor for activated C kinase 1 (RACK1) is involved in autophagosome formation in Drosophila and mice, and that it plays an essential role in morphine-associated memory. However, the exact mechanism of the role of RACK1 in morphine-induced autophagy is not fully understood.

Experimental approach

SH-SY5Y cells were cultured and morphine, rapamycin, 3-methyladenine and RACK1 siRNA were used to evaluate the regulation of RACK1 protein in autophagy. Western blotting and immunofluorescence were used to assess protein expression.

Key results

Activation of autophagy (i.e. autophagosome accumulation and an increase in the LC3-II/LC3

SUBMITTER: Liu LT 

PROVIDER: S-EPMC7060369 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature