Estimating relative biomasses of organisms in microbiota using "phylopeptidomics".

ABSTRACT:

Background

There is an important need for the development of fast and robust methods to quantify the diversity and temporal dynamics of microbial communities in complex environmental samples. Because tandem mass spectrometry allows rapid inspection of protein content, metaproteomics is increasingly used for the phenotypic analysis of microbiota across many fields, including biotechnology, environmental ecology, and medicine.

Results

Here, we present a new method for identifying the biomass contribution of any given organism based on a signature describing the number of peptide sequences shared with all other organisms, calculated by mathematical modeling and phylogenetic relationships. This so-called "phylopeptidomics" principle allows for the calculation of the relative rat

SUBMITTER: Pible O 

PROVIDER: S-EPMC7060547 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature