Late steps in bacterial translation initiation visualized using time-resolved cryo-EM.

ABSTRACT: The initiation of bacterial translation involves the tightly regulated joining of the 50S ribosomal subunit to an initiator transfer RNA (fMet-tRNA^fMet)-containing 30S ribosomal initiation complex to form a 70S initiation complex, which subsequently matures into a 70S elongation-competent complex. Rapid and accurate formation of the 70S initiation complex is promoted by initiation factors, which must dissociate from the 30S initiation complex before the resulting 70S elongation-competent complex can begin the elongation of translation¹. Although comparisons of the structures of the 30S^2-5 and 70S^4,6-8 initiation complexes have revealed that the ribosome, initiation factors and fMet-tRNA^fMet can acquire different conformations in these

SUBMITTER: Kaledhonkar S 

PROVIDER: S-EPMC7060745 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature