Dynamic Lamin B1-Gene Association During Oligodendrocyte Progenitor Differentiation.

ABSTRACT: Differentiation of oligodendrocytes (OL) from progenitor cells (OPC) is the result of a unique program of gene expression, which is further regulated by the formation of topological domains of association with the nuclear lamina. In this study, we show that cultured OPC were characterized by progressively declining levels of endogenous Lamin B1 (LMNB1) during differentiation into OL. We then identify the genes dynamically associated to the nuclear lamina component LMNB1 during this transition, using a well established technique called DamID, which is based on the ability of a bacterially-derived deoxyadenosine methylase (Dam), to modify genomic regions in close proximity. We expressed a fusion protein containing Dam and LMNB1 in OPC (OPC_LMNB1-Dam) and either kept them proliferat

SUBMITTER: Yattah C 

PROVIDER: S-EPMC7060805 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature