Multiple calcium sources are required for intracellular calcium mobilization during gastric organoid epithelial repair.

ABSTRACT: Calcium (Ca²⁺ ) is a known accelerator for gastric wound repair. We have demonstrated in vivo and in vitro that intracellular Ca²⁺ increases in the gastric epithelial cells directly adjacent to a damaged cell, and that this Ca²⁺ rise is essential for the cellular migration that rapidly repairs the epithelium (restitution). While intracellular Ca²⁺ has been shown to be an important signaling factor during epithelial restitution, the source from which this intracellular Ca²⁺ originates remains unclear. Using gastric organoids derived from mice transgenic for a genetically encoded Ca²⁺ indicator, we sought to investigate the potential sources of intracellular Ca²⁺ mobilization. During confocal imaging, photodamage (P

SUBMITTER: Engevik KA 

PROVIDER: S-EPMC7061093 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature