Microbiomes of Endodontic-Periodontal Lesions before and after Chemomechanical Preparation.

ABSTRACT:

Introduction

This study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (CMP).

Methods

Clinical samples were taken from 15 root canals (RCs) with necrotic pulp tissues and from their associated periodontal pockets (PPs) (n = 15) of teeth with endodontic-periodontal lesions before and after CMP. The Human Oral Microbe Identification using Next Generation Sequencing (NGS) protocol and viable culture were used to analyze samples from RCs and PPs. The Mann-Whitney U test and Benjamini-Hochberg corrections were performed to correlate the clinical and radiographic findings with microbial findings (P < .05).

Results

Bacteria were detected in 100% of the samples in both sites (15/15) using NGS. Firmicute

SUBMITTER: Gomes BP 

PROVIDER: S-EPMC7061340 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature