Novel Monoclonal Antibodies Recognizing Human Prostate-Specific Membrane Antigen (PSMA) as Research and Theranostic Tools.

ABSTRACT:

Background

Prostate-specific membrane antigen (PSMA) is a validated target for the imaging and therapy of prostate cancer. Here, we report the detailed characterization of four novel murine monoclonal antibodies (mAbs) recognizing human PSMA as well as PSMA orthologs from different species.

Methods

Performance of purified mAbs was assayed using a comprehensive panel of in vitro experimental setups including Western blotting, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, and surface-plasmon resonance. Furthermore, a mouse xenograft model of prostate cancer was used to compare the suitability of the mAbs for in vivo applications.

Results

All mAbs demonstrate high specificity for PSMA as documented by the lack of cross-reactivity to unrelated human pro

SUBMITTER: Novakova Z 

PROVIDER: S-EPMC7061361 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature