Solid-phase combinatorial synthesis using microarrays of microcompartments with light-induced on-chip cell screening.

ABSTRACT: The process of drug discovery includes individual synthesis and characterization of drug candidates, followed by a biological screening, which is separated from synthesis in space and time. This approach suffers from low throughput and associated high costs, which in turn lead to inefficiency in the field of drug discovery. Here, we present a miniaturized platform combining combinatorial solid-phase synthesis with high-throughput cell screenings. The method is based on the formation of nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) layers patterned with hydrophilic spots separated from each other by superhydrophobic liquid-impermeable barriers. The porous polymer inside the hydrophilic spots is used as a support to conduct solid-phase synthesis. The hydrophilic spo

SUBMITTER: Rosenfeld A 

PROVIDER: S-EPMC7061619 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature