Percutaneous endoscopy in direct real-time observation of choke vessels in rat perforator flap model.

ABSTRACT:

Background

Most of the techniques used to investigate choke vessels are indirect. The aim of the present study is to assess the effectiveness of percutaneous endoscopy in direct real-time visualization of choke vessels in rat perforator flap models.

Methods

A classic perforator flap on the rat dorsum was designed (n = 12). An additional incision was made to place the percutaneous endoscope. Evans blue dye was injected from the common carotid artery to distinguish choke arteries from veins. Blood perfusion status was assessed using full-field laser perfusion imaging (FLPI) and the oxygen/carbon dioxide levels. Photographs of choke vessels were taken and compared at 1 h, 1 day, 4 days, and 7 days postoperation. The flap survival area was examined on day 7.

Results

SUBMITTER: Meng X 

PROVIDER: S-EPMC7061670 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature