Development of an immunoassay for the detection of carbaryl in cereals based on a camelid variable heavy-chain antibody domain.

ABSTRACT:

Background

The variable domain of camelid heavy-chain antibodies (VHH) is increasingly being adapted to detect small molecules in various matrices. The insecticide carbaryl is widely used in agriculture while its residues have posed a threat to food safety and human health.

Results

VHHs specific for carbaryl were generated from an alpaca immunized with the hapten CBR1 coupled to keyhole limpet hemocyanin. An enzyme-linked immunosorbent assay (ELISA) based on the VHH C1 and the coating antigen CBR2-BSA was developed for the detection of carbaryl in cereals. This assay, using an optimized assay buffer (pH 6.5) containing 10% methanol and 0.8% NaCl, has a half-maximum signal inhibition concentration of 5.4 ng mL^-1 and a limit of detection (LOD) of 0.3 ng mL^-1

SUBMITTER: Liu Z 

PROVIDER: S-EPMC7061733 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature