Ultrafast Oxime Formation Enables Efficient Fluorescence Light-up Measurement of DNA Base Excision.

ABSTRACT: DNA glycosylases constitute a biologically and biomedically important group of DNA repair enzymes responsible for initiating base excision repair (BER). Measuring their activities can be useful for studying the mechanisms DNA damage and repair and for practical applications in cancer diagnosis and drug screening. Previous fluorescence methods for assaying DNA glycosylases are often complex and/or limited in scope to a single enzyme type. Here we report a universal base excision reporter (UBER) fluorescence probe design that implements an unprecedentedly rapid oxime reaction (>150 M^-1 s^-1) with high specificity for the abasic (AP) site of DNA. The molecular rotor design achieves a robust >250-500-fold increase in fluorescence upon reaction with AP sites in DNA. By usin

SUBMITTER: Wilson DL 

PROVIDER: S-EPMC7061905 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature