Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction.

ABSTRACT: Antigen binding by antibodies requires precise orientation of the complementarity- determining region (CDR) loops in the variable domain to establish the correct contact surface. Members of the family Camelidae have a modified form of immunoglobulin gamma (IgG) with only heavy chains, called Heavy Chain only Antibodies (HCAb). Antigen binding in HCAbs is mediated by only three CDR loops from the single variable domain (V_HH) at the N-terminus of each heavy chain. This feature of the V_HH, along with their other important features, e.g., easy expression, small size, thermo-stability and hydrophilicity, made them promising candidates for therapeutics and diagnostics. Thus, to design better V_HH domains, it is important to thoroughly understand their sequence and

SUBMITTER: Melarkode Vattekatte A 

PROVIDER: S-EPMC7061911 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature