A novel workflow for three-dimensional analysis of tumour cell migration.

ABSTRACT: The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

SUBMITTER: Ketchen S 

PROVIDER: S-EPMC7061943 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature